COMPARISON OF MICROBIOTA IN THREATENED PRETERM LABOR AND NORMAL PREGNANCY AT RSUP DR.M. DJAMIL PADANG
DOI:
https://doi.org/10.25077/aoj.8.1.554-565.2024Keywords:
Partus Prematurus Iminens, Vaginal microbiotaAbstract
One of the threats in pregnancy is threatened preterm labor. Several studies show that thecomposition of the microbiota in the vagina of premature births and normal pregnancies is different.
This study aims to determine the comparison of microbiota in pregnancies with premature parturition
and normal pregnancies at Dr M Djamil General Hospital, Padang. This research is an analytical
observational study with a case–control study design conducted at the Department/SMF of Obstetrics
and Gynecology, RSUP Dr. M. Djamil Padang from July – September 2023. The study population in this
research were all pregnant women with a gestational age of 20-37 weeks who were diagnosed with
imminens preterm labor and normal pregnant women with a gestational age of 20-37 weeks who
received antenatal care at RSUP Dr. M. Djamil Padang. Respondents had vaginal swabs taken for
microbiological culture at the Biomedical Laboratory, Andalas University, Padang. There were 33
respondents each in the imminence premature birth and normal pregnancy groups. Vaginal swab
results showed that the most common microorganism found in normal pregnancy was Lactobacilus
sp. (73%) while in threatened preterm labor Staphylococcus aureus (37%), Staphylococcus epidermidis
(27%) and E.coli (15%) were more commonly found with only a small proportion of Lactobacilus sp
(12%). Chi square analysis showed a significant difference between the vaginal microbiota of normal
pregnant women and pregnant women with PPI. The conclusion of this study is that there is an
increase in Staphylococcus aureus, Staphylococcus epidermidis and E.coli in pregnant women on PPI
with a decrease in the number of normal flora, namely Lactobacilus spp.
References
Griggs KM, Hrelic DA, Williams N, McEwen-Campbell M, Cypher R. Preterm labor and birth: a
clinical review. MCN Am J Matern Nurs. 2020;45(6):328-337.
Safari S, Hamrah MP. Epidemiology and related risk factors of preterm labor as an obstetrics
emergency. Emergency. 2017;5(1).
Seedat F, Stinton C, Patterson J, et al. Adverse events in women and children who have received
intrapartum antibiotic prophylaxis treatment: a systematic review. BMC Pregnancy Childbirth.
;17(1):1-14.
Nunez N, Réot L, Menu E. Neonatal immune system ontogeny: the role of maternal microbiota
and associated factors. How might the non-human primate model enlighten the path? Vaccines.
;9(6):584.
Brown RG, Marchesi JR, Lee YS, et al. Vaginal dysbiosis increases risk of preterm fetal membrane
rupture, neonatal sepsis and is exacerbated by erythromycin. BMC Med. 2018;16(1):1-15.
Singh A, Kanti V, Dayal S, Shukla SK, Mishra N. Prevalence and risk factors of bacterial vaginosis
among women of reproductive age attending rural tertiary care institute of Western Uttar
Pradesh. J Evol Med Dent Sci. 2016;5(43):2695-2702.
Lamont RF, Sobel JD, Akins RA, et al. The vaginal microbiome: new information about genital tract
flora using molecular based techniques. BJOG An Int J Obstet Gynaecol. 2011;118(5):533-549.
Tamarelle J, Ma B, Gajer P, et al. Nonoptimal vaginal microbiota after azithromycin treatment for
Chlamydia trachomatis infection. J Infect Dis. 2020;221(4):627-635.
Bayar E, Bennett PR, Chan D, Sykes L, MacIntyre DA. The pregnancy microbiome and preterm birth.
In: Seminars in Immunopathology. Vol 42. Springer; 2020:487-499.
Kacerovsky M, Vrbacky F, Kutova R, et al. Cervical microbiota in women with preterm prelabor
rupture of membranes. PLoS One. 2015;10(5):e0126884.
Kamgobe E, Grote S, Mushi MF, et al. Multi-drug resistant facultative pathogenic bacteria
colonizing the vagina of pregnant women with premature rupture of membrane, Tanzania. East
Africa Sci. 2020;2(1):29-35.
Masteryanto HM, Hardianto G, Joewono HT, Koendhori EB. Infeksi Saluran Kemih Sebagai Faktor
Risiko Terjadinya Ancaman Persalinan Preterm. Maj Obstet dan Ginekol. 2015;23(2):75-81.
Giannella L, Grelloni et al. J. Microbiome change on pregnancy disorders. MDPI. 2023.
Hillier SL, Nugent RP, Eschenbach DA, et al. Association between bacterial vaginosis and preterm
delivery of a low-birth-weight infant. N Engl J Med. 1995;333(26):1737-1742.
Aagaard K, Riehle K, Ma J, et al. A metagenomic approach to characterization of the vaginal
microbiome signature in pregnancy. PLoS One. 2012;7(6):e36466.
Hyman RW, Fukushima M, Jiang H, et al. Diversity of the vaginal microbiome correlates with
preterm birth. Reprod Sci. 2014;21(1):32-40.
Kirana P, Islamy N, Yonata A. G4P3A0 hamil 30 minggu belum inpartu dengan partus
prematurus imminens dan ketuban pecah dini. Medula. 2020; 10(3): 456-60.
Widiana I, Putra I, Budiana I, Manuaba I. Karakteristik pasien partus prematurus imminens
di RSUP Sanglah Denpasar periode 1 April 2016-30 September 2017. E-Jurnal Medika.
; 8(3).
Widandi MN, Herdiyantini M, Sudiarta KE. Karakteristik Partus Prematurus Imminens Di
RSPAL Dr Ramelan Surabaya Periode Juni 2019 – Juni 2020. HTMJ, 2022; 19(2): 193-
Yeni CM, Rismawati, Hasanuddin. Ancaman Persalinan Preterm: Mana yang menjadi
Persalinan Preterm?. 2020; 8(4): 216-21.
Fuchs F, Monet B, Ducruet T, Chaillet N, Audibert F. Effect of maternal age on the risk of
preterm birth: A large cohort study. PLoS ONE. 2018; 13(1): e0191002.
Suman V, Luther EE. Preterm Labor. [Updated 2023 Aug 8]. In: StatPearls [Internet].
Treasure Island (FL): StatPearls Publishing; 2023 Jan-. Available from:
https://www.ncbi.nlm.nih.gov/books/NBK536939/
Dudenhausen JW, Kunze M, Wittwer-Backofen U, et al. The relationship between maternal
age, body mass index, and the rate of preterm birth. J Turk Ger Gynecol Assoc.
;19(4):182-186. doi:10.4274/jtgga.2018.0057
Grewal K, MacIntyre DA, Bennett PR. The reproductive tract microbiota in
pregnancy. Biosci Rep. 2021;41(9):BSR20203908. doi:10.1042/BSR20203908,
Li D, Chi X, Zhang L, Chen R, Cao J, Sun X, et al. Vaginal microbiome analysis of healthy
women during different periods of gestation. Biosci Rep. 2020; 40 (7): BSR20201766.
doi: https://doi.org/10.1042/BSR20201766
Gupta P, Singh MP and Goyal K. Diversity of Vaginal Microbiome in Pregnancy:
Deciphering the Obscurity. Front. Public Health. 2020; 8:326. doi:
3389/fpubh.2020.00326
Nunn KL, Witkin SS, Schneider GM, et al. Changes in the Vaginal Microbiome during the Pregnancy
to Postpartum Transition. Reprod Sci. 2021;28(7):1996-2005. doi:10.1007/s43032-020-00438-6
DiGiulio DB, Callahan BJ, McMurdie PJ, et al. Temporal and spatial variation of the human
microbiota during pregnancy. Proc Natl Acad Sci. 2015;112(35):11060-11065.
Mohammadi D, Naghshineh E, Sarsangi A, Zare Sakhvidi MJ. Environmental extreme temperatureand daily preterm birth in Sabzevar, Iran: a time-series analysis. Environ Health Prev Med.
;24(1):1-13.
Vanesha V, Wantania JJE, Lengkong RA. Pola Mikroorganisme Vagina pada Ketuban Pecah Dini.
Indones J Obstet Gynecol. 2021;9(4): 198-203.
Mu’arofah B, Minawa DVP. Deteksi Bakteri Staphylococcus spp. pada Sekret Vagina Ibu Hamil di
RSIA Citra Keluarga Kota Kediri. J. Sintesis. 2021; 2(1): 32-8
Yang S, Reid G, Challis JRG, et al. E ff ect of Oral Probiotic Lactobacillus rhamnosus GR-1. Published
online 2020:1-17.
Kervinen K, Holster T, Saqib S, et al. Parity and gestational age are associated with vaginal
microbiota composition in term and late term pregnancies. EBioMedicine. 2022;81
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