Lactobacillus species and Clamydia trachomatis characterisation in premature rupture of membranes and normal pregnancy
DOI:
https://doi.org/10.25077/aoj.9.1.95-104.2025Keywords:
Lactobacillus vaginalis, chlamydia trachomatis, Lactobacillus crispatus, PROM, normal pregnancyAbstract
The cause of premature rupture of membranes (PROM) remains uncertain. The vaginal microbiota might possess a significant influence on the vaginal environment and can impact the overall health of the female reproductive system.
Objective: This study aims to determine the difference in the concentration of lactobacillus crispatus, lactobacillus vaginalis, and chlamydia trachomatis between patients with premature rupture of membranes and normal pregnancies;
Methods: This study is an analytic study with a case-control design that was conducted at Dr. M. Djamil Hospital Padang during the period January 2024 to June 2024. This study performed DNA isolation using conventional PCR;
Results: Lactobacillus crispatus, Lactobacillus vaginalis, and Chlamydia trachomatis reached 42,124,873.86/mL, 179.41/mL, and 287,062.30/mL, respectively in preterm rupture of membranes patients. In normal pregnancy, Lactobacillus crispatus, Lactobacillus vaginalis, and Chlamydia trachomatis reached 75,717,348.80/mL, 704,147.66/mL, and 0/mL, respectively. A significant difference in Lactobacillus vaginalis concentrations was found between the case and control groups, with the control group having a greater average concentration (28.88) compared to the case group (36.13), with a p-value <0.018. The case group had a significantly greater concentration of Chlamydia trachomatis (37.50) compared to the control group (27.50), with a p-value <0.001. The case and control groups had significantly different lactobacillus crispatus concentrations. The case group had 24.88 times less lactobacillus crispatus than the control group, which had 40.13 times more, with p-value <0.001;
Conclusion: In pregnant women, an increase in Chlamydia trachomatis and a reduction in Lactobacillus crispatus and Lactobacillus vaginalis can be an indicator of probable PROM.
Keywords: Lactobacillus vaginalis, chlamydia trachomatis, Lactobacillus crispatus, PROM, normal pregnancy.
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Putri MF, Haviz Yuad, Andani Eka Putra. Comparison Of Lactobacillus Plantarum, Lactobacillus Mucosae And Lactobacillus Farciminis Bacteria Between Polycystic Ovary Syndrome And Non Polycystic Ovary Syndrome Students At Medical Faculty Of Andalas University. Andalas Obstet Gynecol J. 2024;8(2):732-745. doi:10.25077/aoj.8.2.732-745.2024
Gumilar RW, Utama BI, Bachtiar H. Correlation of Colony Lactobacillus spp. with The Incident of Overactive Bladder with OBSS Score at Pauh Primary Health Center Padang. Andalas Obstet Gynecol J. 2021;5(1):56-62. doi:10.25077/aoj.5.1.56-62.2021
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